Purification and activation of brain sulfotransferase.
نویسندگان
چکیده
منابع مشابه
Purification and characterization of a canine liver phenol sulfotransferase.
Species differences in the metabolism of xenobiotics can present significant problems for safety and efficacy assessment during the development of new pharmaceutical agents. Identification of animal models for human metabolism and/or toxicology of any particular compound would significantly reduce the extent and cost of animal testing of novel candidate pharmaceuticals. Sulfation is an importan...
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N-Hydroxy-2-acetylaminofluorene (N-OH-2-AAF) sulfotransferase is an enzyme that catalyzes the sulfate transfer from the active sulfate, 3'-phosphoadenosine 5'-phosphosulfate (PAPS), to N-OH-2-AAF to form a highly reactive product acetylaminofluorene N-sulfate. It has been purified about 2000-fold with a yield of over 12% from adult Sprague-Dawley male rat livers by an eight-step procedure. The ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1992
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)35942-8